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rat anti human runx2 cbfa1  (R&D Systems)


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    R&D Systems rat anti human runx2 cbfa1
    Rat Anti Human Runx2 Cbfa1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+anti+human+runx2+cbfa1/Human+RUNX2%2FCBFA1+Antibody/pmc12463118__ADVS-12-e14194-s002-89-55-59
    Average 93 stars, based on 39 article reviews
    rat anti human runx2 cbfa1 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: Woven bone formation and mineralization by rat mesenchymal stromal cells imply increased expression of the intermediate filament desmin
    Article Snippet: .. For light microscopic (LM) single-labeling indirect immunofluorescence (IF), cells were initially immunostained using either a polyclonal rabbit anti -DES (1:50, Abcam, UK) for 1h at RT, or a monoclonal mouse anti-vimentin (1:500, clone VIM 13., SIGMA, USA), or a rat anti-human RUNX2/CBFA1 to the isoform 2 (1:30, R&D Systems, USA) cross-reacting with rat, overnight at 4C°. ..

    Article Title: Human Bone‐Derived Endothelial Cells Mediate Bone Regeneration via Distinct Expression of KIT Ligand
    Article Snippet: .. Antibody Vendor Cat number Dilution* Mouse anti-human Vimentin (V9) Abcam Ab8069 1:200 (IF) Rabbit anti-Osterix (OSX) Abcam ab22552 1:200 (IF) Goat anti-human/mouse CD117/c-Kit Novus Biologicals AF1356 1:50 (IF, IHC) Mouse anti-human CD31 (JC70A, human specific) Agilent M082329-2 1:200 (IF) Mouse anti-human CD34 Antibody BioLegend 343608 1:200 (IF) Rabbit anti-human Osteocalcin (OCN) Proteintech 23418-1-AP 1:200 (IF) Rat anti-human RUNX2/CBFA1 (232902) R&D Systems MAB2006 1:200 (IF) Rabbit anti-human Von Willebrand Factor (vWF) DAKO A0082 1:200 (IF) Mouse anti-human Osteocalcin (OCN) R&D Systems MAB1419-SP 1:200 (IF) Rabbit anti-α smooth muscle actin (1A4) Sigma-Aldrich A2547 1:300 (IF) Rhodamine labeled Ulex Europaeus Agglutinin I (UEA I, human specific) Vector Laboratories RL-1062 1:100 (IF) PE anti-human CD90 (Thy1) BioLegend 328109 1:100 (MACS) Texas Red -conjugated horse anti-mouse IgG Vector Laboratories TI-2000 1:200 (IF) FITC-conjugated horse antimouse IgG Vector Laboratories FI-2000 1:200 (IF) Peroxidase-conjugated horse anti-mouse IgG Vector Laboratories PI-2000 1:200 (IHC) Texas Red-conjugated goat anti-rabbit IgG Vector Laboratories TI-1000 1:200 (IF) FITC-conjugated goat antirabbit IgG Vector Laboratories FI-5000 1:200 (IF) Donkey anti-Rat IgG ThermoFisher A78946 1:200 (IF) Donkey anti-Goat IgG ThermoFisher A32814 1:200 (IF) Notch1 (D1E11) XP® Rabbit mAb Cell Signaling Technology 3608S 1:1000 (WB) Human Osteopontin/OPN Antibody R&D Systems MAB14331-100 1:1000 (WB) Anti-GAPDH antibody Millipore Sigma G9545-100UL 1:1000 (WB) Anti-Rabbit-HRP Kindle Biosciences R1004 1:1000 (WB) Anti-Mouse-HRP Kindle Biosciences R1004 1:1000 (WB) * IHC: immunohistochemistry staining; IF: immunofluorescence staining; MACS: magnetic- activated cell sorting; WB: western blot. ..

    IF-cells:

    Article Title: Woven bone formation and mineralization by rat mesenchymal stromal cells imply increased expression of the intermediate filament desmin
    Article Snippet: .. For light microscopic (LM) single-labeling indirect immunofluorescence (IF), cells were initially immunostained using either a polyclonal rabbit anti -DES (1:50, Abcam, UK) for 1h at RT, or a monoclonal mouse anti-vimentin (1:500, clone VIM 13., SIGMA, USA), or a rat anti-human RUNX2/CBFA1 to the isoform 2 (1:30, R&D Systems, USA) cross-reacting with rat, overnight at 4C°. ..

    Immunohistochemistry:

    Article Title: Human Bone‐Derived Endothelial Cells Mediate Bone Regeneration via Distinct Expression of KIT Ligand
    Article Snippet: .. Antibody Vendor Cat number Dilution* Mouse anti-human Vimentin (V9) Abcam Ab8069 1:200 (IF) Rabbit anti-Osterix (OSX) Abcam ab22552 1:200 (IF) Goat anti-human/mouse CD117/c-Kit Novus Biologicals AF1356 1:50 (IF, IHC) Mouse anti-human CD31 (JC70A, human specific) Agilent M082329-2 1:200 (IF) Mouse anti-human CD34 Antibody BioLegend 343608 1:200 (IF) Rabbit anti-human Osteocalcin (OCN) Proteintech 23418-1-AP 1:200 (IF) Rat anti-human RUNX2/CBFA1 (232902) R&D Systems MAB2006 1:200 (IF) Rabbit anti-human Von Willebrand Factor (vWF) DAKO A0082 1:200 (IF) Mouse anti-human Osteocalcin (OCN) R&D Systems MAB1419-SP 1:200 (IF) Rabbit anti-α smooth muscle actin (1A4) Sigma-Aldrich A2547 1:300 (IF) Rhodamine labeled Ulex Europaeus Agglutinin I (UEA I, human specific) Vector Laboratories RL-1062 1:100 (IF) PE anti-human CD90 (Thy1) BioLegend 328109 1:100 (MACS) Texas Red -conjugated horse anti-mouse IgG Vector Laboratories TI-2000 1:200 (IF) FITC-conjugated horse antimouse IgG Vector Laboratories FI-2000 1:200 (IF) Peroxidase-conjugated horse anti-mouse IgG Vector Laboratories PI-2000 1:200 (IHC) Texas Red-conjugated goat anti-rabbit IgG Vector Laboratories TI-1000 1:200 (IF) FITC-conjugated goat antirabbit IgG Vector Laboratories FI-5000 1:200 (IF) Donkey anti-Rat IgG ThermoFisher A78946 1:200 (IF) Donkey anti-Goat IgG ThermoFisher A32814 1:200 (IF) Notch1 (D1E11) XP® Rabbit mAb Cell Signaling Technology 3608S 1:1000 (WB) Human Osteopontin/OPN Antibody R&D Systems MAB14331-100 1:1000 (WB) Anti-GAPDH antibody Millipore Sigma G9545-100UL 1:1000 (WB) Anti-Rabbit-HRP Kindle Biosciences R1004 1:1000 (WB) Anti-Mouse-HRP Kindle Biosciences R1004 1:1000 (WB) * IHC: immunohistochemistry staining; IF: immunofluorescence staining; MACS: magnetic- activated cell sorting; WB: western blot. ..

    Labeling:

    Article Title: Human Bone‐Derived Endothelial Cells Mediate Bone Regeneration via Distinct Expression of KIT Ligand
    Article Snippet: .. Antibody Vendor Cat number Dilution* Mouse anti-human Vimentin (V9) Abcam Ab8069 1:200 (IF) Rabbit anti-Osterix (OSX) Abcam ab22552 1:200 (IF) Goat anti-human/mouse CD117/c-Kit Novus Biologicals AF1356 1:50 (IF, IHC) Mouse anti-human CD31 (JC70A, human specific) Agilent M082329-2 1:200 (IF) Mouse anti-human CD34 Antibody BioLegend 343608 1:200 (IF) Rabbit anti-human Osteocalcin (OCN) Proteintech 23418-1-AP 1:200 (IF) Rat anti-human RUNX2/CBFA1 (232902) R&D Systems MAB2006 1:200 (IF) Rabbit anti-human Von Willebrand Factor (vWF) DAKO A0082 1:200 (IF) Mouse anti-human Osteocalcin (OCN) R&D Systems MAB1419-SP 1:200 (IF) Rabbit anti-α smooth muscle actin (1A4) Sigma-Aldrich A2547 1:300 (IF) Rhodamine labeled Ulex Europaeus Agglutinin I (UEA I, human specific) Vector Laboratories RL-1062 1:100 (IF) PE anti-human CD90 (Thy1) BioLegend 328109 1:100 (MACS) Texas Red -conjugated horse anti-mouse IgG Vector Laboratories TI-2000 1:200 (IF) FITC-conjugated horse antimouse IgG Vector Laboratories FI-2000 1:200 (IF) Peroxidase-conjugated horse anti-mouse IgG Vector Laboratories PI-2000 1:200 (IHC) Texas Red-conjugated goat anti-rabbit IgG Vector Laboratories TI-1000 1:200 (IF) FITC-conjugated goat antirabbit IgG Vector Laboratories FI-5000 1:200 (IF) Donkey anti-Rat IgG ThermoFisher A78946 1:200 (IF) Donkey anti-Goat IgG ThermoFisher A32814 1:200 (IF) Notch1 (D1E11) XP® Rabbit mAb Cell Signaling Technology 3608S 1:1000 (WB) Human Osteopontin/OPN Antibody R&D Systems MAB14331-100 1:1000 (WB) Anti-GAPDH antibody Millipore Sigma G9545-100UL 1:1000 (WB) Anti-Rabbit-HRP Kindle Biosciences R1004 1:1000 (WB) Anti-Mouse-HRP Kindle Biosciences R1004 1:1000 (WB) * IHC: immunohistochemistry staining; IF: immunofluorescence staining; MACS: magnetic- activated cell sorting; WB: western blot. ..

    Magnetic Cell Separation:

    Article Title: Human Bone‐Derived Endothelial Cells Mediate Bone Regeneration via Distinct Expression of KIT Ligand
    Article Snippet: .. Antibody Vendor Cat number Dilution* Mouse anti-human Vimentin (V9) Abcam Ab8069 1:200 (IF) Rabbit anti-Osterix (OSX) Abcam ab22552 1:200 (IF) Goat anti-human/mouse CD117/c-Kit Novus Biologicals AF1356 1:50 (IF, IHC) Mouse anti-human CD31 (JC70A, human specific) Agilent M082329-2 1:200 (IF) Mouse anti-human CD34 Antibody BioLegend 343608 1:200 (IF) Rabbit anti-human Osteocalcin (OCN) Proteintech 23418-1-AP 1:200 (IF) Rat anti-human RUNX2/CBFA1 (232902) R&D Systems MAB2006 1:200 (IF) Rabbit anti-human Von Willebrand Factor (vWF) DAKO A0082 1:200 (IF) Mouse anti-human Osteocalcin (OCN) R&D Systems MAB1419-SP 1:200 (IF) Rabbit anti-α smooth muscle actin (1A4) Sigma-Aldrich A2547 1:300 (IF) Rhodamine labeled Ulex Europaeus Agglutinin I (UEA I, human specific) Vector Laboratories RL-1062 1:100 (IF) PE anti-human CD90 (Thy1) BioLegend 328109 1:100 (MACS) Texas Red -conjugated horse anti-mouse IgG Vector Laboratories TI-2000 1:200 (IF) FITC-conjugated horse antimouse IgG Vector Laboratories FI-2000 1:200 (IF) Peroxidase-conjugated horse anti-mouse IgG Vector Laboratories PI-2000 1:200 (IHC) Texas Red-conjugated goat anti-rabbit IgG Vector Laboratories TI-1000 1:200 (IF) FITC-conjugated goat antirabbit IgG Vector Laboratories FI-5000 1:200 (IF) Donkey anti-Rat IgG ThermoFisher A78946 1:200 (IF) Donkey anti-Goat IgG ThermoFisher A32814 1:200 (IF) Notch1 (D1E11) XP® Rabbit mAb Cell Signaling Technology 3608S 1:1000 (WB) Human Osteopontin/OPN Antibody R&D Systems MAB14331-100 1:1000 (WB) Anti-GAPDH antibody Millipore Sigma G9545-100UL 1:1000 (WB) Anti-Rabbit-HRP Kindle Biosciences R1004 1:1000 (WB) Anti-Mouse-HRP Kindle Biosciences R1004 1:1000 (WB) * IHC: immunohistochemistry staining; IF: immunofluorescence staining; MACS: magnetic- activated cell sorting; WB: western blot. ..

    Western Blot:

    Article Title: Human Bone‐Derived Endothelial Cells Mediate Bone Regeneration via Distinct Expression of KIT Ligand
    Article Snippet: .. Antibody Vendor Cat number Dilution* Mouse anti-human Vimentin (V9) Abcam Ab8069 1:200 (IF) Rabbit anti-Osterix (OSX) Abcam ab22552 1:200 (IF) Goat anti-human/mouse CD117/c-Kit Novus Biologicals AF1356 1:50 (IF, IHC) Mouse anti-human CD31 (JC70A, human specific) Agilent M082329-2 1:200 (IF) Mouse anti-human CD34 Antibody BioLegend 343608 1:200 (IF) Rabbit anti-human Osteocalcin (OCN) Proteintech 23418-1-AP 1:200 (IF) Rat anti-human RUNX2/CBFA1 (232902) R&D Systems MAB2006 1:200 (IF) Rabbit anti-human Von Willebrand Factor (vWF) DAKO A0082 1:200 (IF) Mouse anti-human Osteocalcin (OCN) R&D Systems MAB1419-SP 1:200 (IF) Rabbit anti-α smooth muscle actin (1A4) Sigma-Aldrich A2547 1:300 (IF) Rhodamine labeled Ulex Europaeus Agglutinin I (UEA I, human specific) Vector Laboratories RL-1062 1:100 (IF) PE anti-human CD90 (Thy1) BioLegend 328109 1:100 (MACS) Texas Red -conjugated horse anti-mouse IgG Vector Laboratories TI-2000 1:200 (IF) FITC-conjugated horse antimouse IgG Vector Laboratories FI-2000 1:200 (IF) Peroxidase-conjugated horse anti-mouse IgG Vector Laboratories PI-2000 1:200 (IHC) Texas Red-conjugated goat anti-rabbit IgG Vector Laboratories TI-1000 1:200 (IF) FITC-conjugated goat antirabbit IgG Vector Laboratories FI-5000 1:200 (IF) Donkey anti-Rat IgG ThermoFisher A78946 1:200 (IF) Donkey anti-Goat IgG ThermoFisher A32814 1:200 (IF) Notch1 (D1E11) XP® Rabbit mAb Cell Signaling Technology 3608S 1:1000 (WB) Human Osteopontin/OPN Antibody R&D Systems MAB14331-100 1:1000 (WB) Anti-GAPDH antibody Millipore Sigma G9545-100UL 1:1000 (WB) Anti-Rabbit-HRP Kindle Biosciences R1004 1:1000 (WB) Anti-Mouse-HRP Kindle Biosciences R1004 1:1000 (WB) * IHC: immunohistochemistry staining; IF: immunofluorescence staining; MACS: magnetic- activated cell sorting; WB: western blot. ..

    Staining:

    Article Title: Human Bone‐Derived Endothelial Cells Mediate Bone Regeneration via Distinct Expression of KIT Ligand
    Article Snippet: .. Antibody Vendor Cat number Dilution* Mouse anti-human Vimentin (V9) Abcam Ab8069 1:200 (IF) Rabbit anti-Osterix (OSX) Abcam ab22552 1:200 (IF) Goat anti-human/mouse CD117/c-Kit Novus Biologicals AF1356 1:50 (IF, IHC) Mouse anti-human CD31 (JC70A, human specific) Agilent M082329-2 1:200 (IF) Mouse anti-human CD34 Antibody BioLegend 343608 1:200 (IF) Rabbit anti-human Osteocalcin (OCN) Proteintech 23418-1-AP 1:200 (IF) Rat anti-human RUNX2/CBFA1 (232902) R&D Systems MAB2006 1:200 (IF) Rabbit anti-human Von Willebrand Factor (vWF) DAKO A0082 1:200 (IF) Mouse anti-human Osteocalcin (OCN) R&D Systems MAB1419-SP 1:200 (IF) Rabbit anti-α smooth muscle actin (1A4) Sigma-Aldrich A2547 1:300 (IF) Rhodamine labeled Ulex Europaeus Agglutinin I (UEA I, human specific) Vector Laboratories RL-1062 1:100 (IF) PE anti-human CD90 (Thy1) BioLegend 328109 1:100 (MACS) Texas Red -conjugated horse anti-mouse IgG Vector Laboratories TI-2000 1:200 (IF) FITC-conjugated horse antimouse IgG Vector Laboratories FI-2000 1:200 (IF) Peroxidase-conjugated horse anti-mouse IgG Vector Laboratories PI-2000 1:200 (IHC) Texas Red-conjugated goat anti-rabbit IgG Vector Laboratories TI-1000 1:200 (IF) FITC-conjugated goat antirabbit IgG Vector Laboratories FI-5000 1:200 (IF) Donkey anti-Rat IgG ThermoFisher A78946 1:200 (IF) Donkey anti-Goat IgG ThermoFisher A32814 1:200 (IF) Notch1 (D1E11) XP® Rabbit mAb Cell Signaling Technology 3608S 1:1000 (WB) Human Osteopontin/OPN Antibody R&D Systems MAB14331-100 1:1000 (WB) Anti-GAPDH antibody Millipore Sigma G9545-100UL 1:1000 (WB) Anti-Rabbit-HRP Kindle Biosciences R1004 1:1000 (WB) Anti-Mouse-HRP Kindle Biosciences R1004 1:1000 (WB) * IHC: immunohistochemistry staining; IF: immunofluorescence staining; MACS: magnetic- activated cell sorting; WB: western blot. ..

    FACS:

    Article Title: Human Bone‐Derived Endothelial Cells Mediate Bone Regeneration via Distinct Expression of KIT Ligand
    Article Snippet: .. Antibody Vendor Cat number Dilution* Mouse anti-human Vimentin (V9) Abcam Ab8069 1:200 (IF) Rabbit anti-Osterix (OSX) Abcam ab22552 1:200 (IF) Goat anti-human/mouse CD117/c-Kit Novus Biologicals AF1356 1:50 (IF, IHC) Mouse anti-human CD31 (JC70A, human specific) Agilent M082329-2 1:200 (IF) Mouse anti-human CD34 Antibody BioLegend 343608 1:200 (IF) Rabbit anti-human Osteocalcin (OCN) Proteintech 23418-1-AP 1:200 (IF) Rat anti-human RUNX2/CBFA1 (232902) R&D Systems MAB2006 1:200 (IF) Rabbit anti-human Von Willebrand Factor (vWF) DAKO A0082 1:200 (IF) Mouse anti-human Osteocalcin (OCN) R&D Systems MAB1419-SP 1:200 (IF) Rabbit anti-α smooth muscle actin (1A4) Sigma-Aldrich A2547 1:300 (IF) Rhodamine labeled Ulex Europaeus Agglutinin I (UEA I, human specific) Vector Laboratories RL-1062 1:100 (IF) PE anti-human CD90 (Thy1) BioLegend 328109 1:100 (MACS) Texas Red -conjugated horse anti-mouse IgG Vector Laboratories TI-2000 1:200 (IF) FITC-conjugated horse antimouse IgG Vector Laboratories FI-2000 1:200 (IF) Peroxidase-conjugated horse anti-mouse IgG Vector Laboratories PI-2000 1:200 (IHC) Texas Red-conjugated goat anti-rabbit IgG Vector Laboratories TI-1000 1:200 (IF) FITC-conjugated goat antirabbit IgG Vector Laboratories FI-5000 1:200 (IF) Donkey anti-Rat IgG ThermoFisher A78946 1:200 (IF) Donkey anti-Goat IgG ThermoFisher A32814 1:200 (IF) Notch1 (D1E11) XP® Rabbit mAb Cell Signaling Technology 3608S 1:1000 (WB) Human Osteopontin/OPN Antibody R&D Systems MAB14331-100 1:1000 (WB) Anti-GAPDH antibody Millipore Sigma G9545-100UL 1:1000 (WB) Anti-Rabbit-HRP Kindle Biosciences R1004 1:1000 (WB) Anti-Mouse-HRP Kindle Biosciences R1004 1:1000 (WB) * IHC: immunohistochemistry staining; IF: immunofluorescence staining; MACS: magnetic- activated cell sorting; WB: western blot. ..



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    Image Search Results


    Journal: eLife

    Article Title: Induction of osteogenesis by bone-targeted Notch activation

    doi: 10.7554/eLife.60183

    Figure Lengend Snippet:

    Article Snippet: The following primary antibodies were used: rat anti-Endomucin (SC-65495, Santa Cruz, 1:100), rat anti-PECAM-1 (553370, Pharmingen), rabbit anti-Osterix (Abcam, ab22552, 1:1000), rat anti-Runx2 (MAB2006, R&D, 1:50), rabbit anti-Collagen type I (AB675P, Millipore, 1:200), goat anti-Osteopontin (AF808, R&D Systems, 1:100), rabbit anti-Osteocalcin (LS-C17044, LifeSpan BioSciences, 1:100), goat anti-Dll4 (AF1389, R&D Systems, 1:100), goat anti-Jag1 (J4127, Sigma, 1:100), goat anti-PDGFRβ (AF1042, R&D, 1:100), rabbit anti-NG2 (AB5320, Millipore), goat anti-BCAM (AF8299, R&D Systems, 1:50), rabbit anti-ATP6V1B1+ ATP6V1B2 (ab200839, Abcam), rabbit anti-Aggrecan (AB1031, Millipore), goat anti-Sox9 (AF3075, R&D Systems), rabbit anti-Perilipin (9349, Cell Signaling).

    Techniques: Recombinant, Variant Assay, Plasmid Preparation, Sequencing, Control, Gene Expression, Bicinchoninic Acid Protein Assay, cDNA Synthesis, Amplification, Magnetic Beads, Protease Inhibitor, Western Blot, Software, Staining